Communication between N terminus and loop2 tunes Orai activation.

ABSTRACT: Ca²⁺ release-activated Ca²⁺ (CRAC) channels constitute the major Ca²⁺ entry pathway into the cell. They are fully reconstituted via intermembrane coupling of the Ca²⁺-selective Orai channel and the Ca²⁺-sensing protein STIM1. In addition to the Orai C terminus, the main coupling site for STIM1, the Orai N terminus is indispensable for Orai channel gating. Although the extended transmembrane Orai N-terminal region (Orai1 amino acids 73-91; Orai3 amino acids 48-65) is fully conserved in the Orai1 and Orai3 isoforms, Orai3 tolerates larger N-terminal truncations than Orai1 in retaining store-operated activation. In an attempt to uncover the reason for these isoform-specific structural requirements, we analyzed a series of Orai mutants and

SUBMITTER: Fahrner M 

PROVIDER: S-EPMC5787804 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature