Secreted heme peroxidase from <i>Dictyostelium discoideum</i>: Insights into catalysis, structure, and biological role.

ABSTRACT: Oxidation of halides and thiocyanate by heme peroxidases to antimicrobial oxidants is an important cornerstone in the innate immune system of mammals. Interestingly, phylogenetic and physiological studies suggest that homologous peroxidases are already present in mycetozoan eukaryotes such as Dictyostelium discoideum This social amoeba kills bacteria via phagocytosis for nutrient acquisition at its single-cell stage and for antibacterial defense at its multicellular stages. Here, we demonstrate that peroxidase A from D. discoideum (DdPoxA) is a stable, monomeric, glycosylated, and secreted heme peroxidase with homology to mammalian peroxidases. The first crystal structure (2.5 Å resolution) of a mycetozoan peroxidase of this superfamily shows the presence of a post-translatio

SUBMITTER: Nicolussi A 

PROVIDER: S-EPMC5787809 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature