A feedback regulatory model for RifQ-mediated repression of rifamycin export in Amycolatopsis mediterranei.

ABSTRACT:

Background

Due to the important role of rifamycin in curing tuberculosis infection, the study on rifamycin has never been stopped. Although RifZ, which locates within the rifamycin biosynthetic cluster, has recently been characterized as a pathway-specific regulator for rifamycin biosynthesis, little is known about the regulation of rifamycin export.

Results

In this work, we proved that the expression of the rifamycin efflux pump (RifP) was regulated by RifQ, a TetR-family transcriptional regulator. Deletion of rifQ had little impact on bacterial growth, but resulted in improved rifamycin production, which was consistent with the reverse transcription PCR results that RifQ negatively regulated rifP's transcription. With electrophoretic mobility shift assay and DNase I Footpr

SUBMITTER: Lei C 

PROVIDER: S-EPMC5787919 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature