Visualizing Dynamics of Cell Signaling In Vivo with a Phase Separation-Based Kinase Reporter.

ABSTRACT: Visualizing dynamics of kinase activity in living animals is essential for mechanistic understanding of cell and developmental biology. We describe GFP-based kinase reporters that phase-separate upon kinase activation via multivalent protein-protein interactions, forming intensively fluorescent droplets. Called SPARK (separation of phases-based activity reporter of kinase), these reporters have large dynamic range (fluorescence change), high brightness, fast kinetics, and are reversible. The SPARK-based protein kinase A (PKA) reporter reveals oscillatory dynamics of PKA activities upon G protein-coupled receptor activation. The SPARK-based extracellular signal-regulated kinase (ERK) reporter unveils transient dynamics of ERK activity during tracheal metamorphosis in live Drosophila. Becaus

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC5788022 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature