Rapid Detection and Identification of Uveitis Pathogens by Qualitative Multiplex Real-Time PCR.

ABSTRACT:

Purpose

Infectious uveitis is a serious sight-threatening infection commonly caused by herpesviruses and Toxoplasma gondii. Etiologic diagnosis based on the clinical evaluation is often challenging. We developed and validated a multiplex real-time PCR assay coupled with high-resolution melting (HRM) for rapid detection and identification of herpes simplex viruses 1 and 2 (HSV-1 and HSV-2), varicella-zoster virus (VZV), cytomegalovirus (CMV), and T. gondii.

Methods

The assay was designed to target pathogen genome regions that yield products with distinct melting temperatures. Analytical specificity, sensitivity, and precision of HRM identification were determined. Clinical validation was performed by testing 108 intraocular fluids collected from eyes suffering with infectious

SUBMITTER: Bispo PJM 

PROVIDER: S-EPMC5788046 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature