Recellularization of rat liver: An in vitro model for assessing human drug metabolism and liver biology.

ABSTRACT: Liver-like organoids that recapitulate the complex functions of the whole liver by combining cells, scaffolds, and mechanical or chemical cues are becoming important models for studying liver biology and drug metabolism. The advantages of growing cells in three-dimensional constructs include enhanced cell-cell and cell-extracellular matrix interactions and preserved cellular phenotype including, prevention of de-differentiation. In the current study, biomimetic liver constructs were made via perfusion decellularization of rat liver, with the goal of maintaining the native composition and structure of the extracellular matrix. We optimized our decellularization process to produce liver scaffolds in which immunogenic residual DNA was removed but glycosaminoglycans were maintained. When the c

SUBMITTER: Robertson MJ 

PROVIDER: S-EPMC5788381 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature