Targeted deep sequencing of circulating tumor DNA in metastatic pancreatic cancer.

ABSTRACT:

Purpose

Precision medicine in pancreatic ductal adenocarcinoma (PDAC) could be substantially supported by tools that allow to establish and monitor the molecular setup of the tumor. In particular, noninvasive approaches are desirable, but not validated. Characterization of circulating tumor DNA (ctDNA) may help to achieve this goal.

Experimental design

Blood samples from patients with metastatic PDAC prior to and during palliative treatment were collected. ctDNA and corresponding tumor tissue were analyzed by targeted next generation sequencing and droplet digital PCR for the 7 most frequently mutated genes in PDAC (TP53, SMAD4, CDKN2A, KRAS, APC, ATM, and FBXW7). Findings were correlated with clinical and imaging data.

Results

A total of 20 patients (therapy naïve

SUBMITTER: Berger AW 

PROVIDER: S-EPMC5788622 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature