Characterization of the effects of defined, multidimensional culture conditions on conditionally reprogrammed primary human prostate cells.

ABSTRACT: The inability to propagate human prostate epithelial cells indefinitely has historically presented a serious impediment to prostate cancer research. The conditionally reprogrammed cell (CRC) approach uses the combination of irradiated J2 mouse fibroblasts and a Rho kinase inhibitor such as Y27632 to support the continuous culture of cells derived from most epithelial tissues, including the prostate. Due to their rapid establishment and overall ease of use, CRCs are now widely used in a variety of basic and preclinical settings. In addition, CRCs were successfully used to clinically treat respiratory papillomatosis. Although both normal and tumor-derived prostate CRCs have been used to study the basic biology of prostate cancer and to test new therapies, certain limitations exist. We have p

SUBMITTER: Tricoli L 

PROVIDER: S-EPMC5788632 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature