Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation.

ABSTRACT: Epithelial cell adhesion molecule EpCAM is expressed in pluripotent embryonic stem cells (ESC) in vitro, but is repressed in differentiated cells, except epithelia and carcinomas. Molecular functions of EpCAM, possibly imposing such repression, were primarily studied in malignant cells and might not apply to non-pathologic differentiation. Here, we comprehensively describe timing and rationale for EpCAM regulation in early murine gastrulation and ESC differentiation using single cell RNA-sequencing datasets, in vivo and in vitro models including CRISPR-Cas9-engineered ESC-mutants. We demonstrate expression of EpCAM in inner cell mass, epiblast, primitive/visceral endoderm, and strict repression in the most primitive, nascent Flk1⁺ mesoderm progenitors at E7.0. Selective expressi

SUBMITTER: Sarrach S 

PROVIDER: S-EPMC5789065 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature