Reagent Tracker Dyes Permit Quality Control for Verifying Plating Accuracy in ELISPOT Tests.

ABSTRACT: ELISPOT assays enable the detection of the frequency of antigen-specific T cells in the blood by measuring the secretion of cytokines, or combinations of cytokines, in response to antigenic challenges of a defined population of PBMC. As such, these assays are suited to establish the magnitude and quality of T cell immunity in infectious, allergic, autoimmune and transplant settings, as well as for measurements of anti-tumor immunity. The simplicity, robustness, cost-effectiveness and scalability of ELISPOT renders it suitable for regulated immune monitoring. In response to the regulatory requirements of clinical and pre-clinical immune monitoring trials, tamper-proof audit trails have been introduced to all steps of ELISPOT analysis: from capturing the raw images of assay wells and countin

SUBMITTER: Lehmann A 

PROVIDER: S-EPMC5789276 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature