Full genome characterization of porcine circovirus type 3 isolates reveals the existence of two distinct groups of virus strains.

ABSTRACT:

Background

The occurrence of the novel porcine circovirus type 3 (PCV3) was reported from the Americas, Asia and Europe. Although this virus was detected in association with various clinical syndromes in pigs, its role as possible swine pathogen remains unclear. PCV3 was detected with high prevalence in Polish farms, but to date no genome sequences were available from European PCV3 strains.

Methods

We collected 1060 serum samples from piglets at the age of 20-24 weeks from 53 farms distributed all over Germany. PCV3 DNA was detected using a real-time PCR and subsequently complete PCV3 genome sequences were obtained after multiply primed rolling circle amplification and sequencing of overlapping PCR products. Phylogenetic analysis was performed by neighbor-joining method and

SUBMITTER: Fux R 

PROVIDER: S-EPMC5789634 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature