Optimized DNA electroporation for primary human T cell engineering.

ABSTRACT:

Background

Effective gene-delivery systems for primary human T cell engineering are useful tools for both basic research and clinical immunotherapy applications. Pseudovirus-based systems and electro-transfection are the most popular strategies for genetic material transduction. Compared with viral-particle-mediated approaches, electro-transfection is theoretically safer, because it does not promote transgene integration into the host genome. Additionally, the simplicity and speed of the procedure increases the attractiveness of electroporation. Here, we developed and optimized an electro-transfection method for the production of engineered chimeric antigen receptor (CAR)-T cells.

Results

Stimulation of T cells had the greatest effect on their transfection, with stimulation

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC5789706 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature