Two novel deep-sea sediment metagenome-derived esterases: residue 199 is the determinant of substrate specificity and preference.

ABSTRACT:

Background

The deep-sea environment harbors a vast pool of novel enzymes. Owing to the limitations of cultivation, cultivation-independent has become an effective method for mining novel enzymes from the environment. Based on a deep-sea sediment metagenomics library, lipolytic-positive clones were obtained by activity-based screening methods.

Results

Two novel esterases, DMWf18-543 and DMWf18-558, were obtained from a deep-sea metagenomic library through activity-based screening and high-throughput sequencing methods. These esterases shared 80.7% amino acid identity with each other and were determined to be new members of bacterial lipolytic enzyme family IV. The two enzymes showed the highest activities toward p-nitrophenyl (p-NP) butyrate at pH 7.0 and 35-40 °C and were fo

SUBMITTER: Huo YY 

PROVIDER: S-EPMC5789746 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature