Significance of Liquid Biopsy for Monitoring and Therapy Decision of Colorectal Cancer.

ABSTRACT:

Purpose

Despite therapeutic improvements, all patients with nonresectable metastatic colorectal cancer (mCRC) acquire resistance to treatment probably due to the growth of mutated clones. In contrast to tissue-based studies, liquid biopsies have enabled the opportunity to reveal emerging resistance to treatment by detecting mutated clones and noninvasively monitoring clonal dynamics during therapy.

Methods

The courses of three patients with mCRC who were initially RAS wild-type were monitored longitudinally using liquid biopsy with long-term follow-up of up to 20 sequential samples. Detection of fragmented RAS mutated circulating cell-free DNA (cf)DNA in plasma was performed by BEAMing. In addition, plasma digital droplet PCR was used to detect and quantify BRAF and PIK3CA m

SUBMITTER: Klein-Scory S 

PROVIDER: S-EPMC5789760 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature