Deciphering microvascular changes after myocardial infarction through 3D fully automated image analysis.

ABSTRACT: The microvasculature continuously adapts in response to pathophysiological conditions to meet tissue demands. Quantitative assessment of the dynamic changes in the coronary microvasculature is therefore crucial in enhancing our knowledge regarding the impact of cardiovascular diseases in tissue perfusion and in developing efficient angiotherapies. Using confocal microscopy and thick tissue sections, we developed a 3D fully automated pipeline that allows to precisely reconstruct the microvasculature and to extract parameters that quantify all its major features, its relation to smooth muscle actin positive cells and capillary diffusion regions. The novel pipeline was applied in the analysis of the coronary microvasculature from healthy tissue and tissue at various stages after myocardial in

SUBMITTER: Gkontra P 

PROVIDER: S-EPMC5789835 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature