Convergent and Divergent Migratory Patterns of Human Neutrophils inside Microfluidic Mazes.

ABSTRACT: Neutrophils are key cellular components of the innate immune response and characteristically migrate from the blood towards and throughout tissues. Their migratory process is complex, guided by multiple chemoattractants released from injured tissues and microbes. How neutrophils integrate the various signals in the tissue microenvironment and mount effective responses is not fully understood. Here, we employed microfluidic mazes that replicate features of interstitial spaces and chemoattractant gradients within tissues to analyze the migration patterns of human neutrophils. We find that neutrophils respond to LTB4 and fMLF gradients with highly directional migration patterns and converge towards the source of chemoattractant. We named this directed migration pattern convergent. Moreover, n

SUBMITTER: Boneschansker L 

PROVIDER: S-EPMC5789854 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature