Role of P2X₄ Receptor in Mouse Voiding Function.

ABSTRACT: Purinergic signalling plays an important role in the regulation of bladder smooth muscle (BSM) contractility, and P2X₄ receptor is expressed in the bladder wall, where it may act by forming heteromeric receptors with P2X₁, the major purinergic force-generating muscle receptor. To test this hypothesis, we examined mouse BSM contractile properties in the absence and presence of selective P2X₁ (NF449 & NF279) and P2X₄ antagonists (5-BDBD). These drugs inhibited BSM purinergic contraction only partially, suggesting the possibility of a heteromeric receptor. However, carefully controlled co-immunoprecipitation experiments indicated that P2X₁ and P2X₄ do not form physically linked heteromers. Furthermore, immunofluorescence staini

SUBMITTER: Yu W 

PROVIDER: S-EPMC5789870 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature