Generation and Validation of the iKp1289 Metabolic Model for Klebsiella pneumoniae KPPR1.

ABSTRACT: Klebsiella pneumoniae has a reputation for causing a wide range of infectious conditions, with numerous highly virulent and antibiotic-resistant strains. Metabolic models have the potential to provide insights into the growth behavior, nutrient requirements, essential genes, and candidate drug targets in these strains. Here we develop a metabolic model for KPPR1, a highly virulent strain of K. pneumoniae. We apply a combination of Biolog phenotype data and fitness data to validate and refine our KPPR1 model. The final model displays a predictive accuracy of 75% in identifying potential carbon and nitrogen sources for K. pneumoniae and of 99% in predicting nonessential genes in rich media. We demonstrate how this model is useful in studying the differences in the metabolic capabilities of t

SUBMITTER: Henry CS 

PROVIDER: S-EPMC5790149 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature