Downregulation of N⁶-methyladenosine binding YTHDF2 protein mediated by miR-493-3p suppresses prostate cancer by elevating N⁶-methyladenosine levels.

ABSTRACT: Recent evidence suggests that m6A modifications regulate the progressions of several types of tumors. YTHDF2, an m6A reader, has been implicated in the regulation of hepatocellular carcinoma (HCC). miR-493-3p has been defined as tumor suppressor that inhibits the progressions of several types of cancers. However, the functions and mechanisms of YTHDF2 and the indirect m6A regulated role of miR-493-3p in prostate cancer (PCa) remains to be elusive. In this study, immuno-histochemical (IHC) staining and chromogenic in situ hybridization (CISH) were performed to find YTHDF2 was frequently upregulated but miR-493-3p was downregulated in both PCa tissues and cell lines (DU-145 and PC3) which was negatively correlated with each other. Knock down of YTHDF2 significantly elevated m6A levels, and i

SUBMITTER: Li J 

PROVIDER: S-EPMC5790497 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature