Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA.

ABSTRACT: We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. The sensitivity of LAMP or PCR was 2.3 or 23CFU for pure culture, whereas 2.3×10⁴ or 2.3×10⁶CFU for simulated spleen and lung samples. For simulated liver samples, the sensitivity of LAMP was 2.3×10⁶CFU, but PCR was negative at the level of 2.3×10⁷CFU. After simulated spleen and lung samples were treated with magnetic beads, the sensitivity of LAMP or PCR was 2.3×10³ or 2.3×10⁶CFU, whereas 2.3×10⁵ or 2.3×10⁷CFU

SUBMITTER: Feng N 

PROVIDER: S-EPMC5790586 | biostudies-literature | 2018 Jan - Mar

REPOSITORIES: biostudies-literature