Zika detection: comparison of methodologies.

ABSTRACT: Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan^® RT-qPCR assay. We used a SYBR^® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan^® RT-qPCR assay, 100% (Kappa=0.670) were also found to be negative by SYBR^® Green RT-qPCR based on Tm comparison; howeve

SUBMITTER: Colombo TE 

PROVIDER: S-EPMC5790641 | biostudies-literature | 2018 Jan - Mar

REPOSITORIES: biostudies-literature