Conditional Protein Splicing Switch in Hyperthermophiles through an Intein-Extein Partnership.

ABSTRACT: Inteins are intervening proteins that undergo an autocatalytic splicing reaction that ligates flanking host protein sequences termed exteins. Some intein-containing proteins have evolved to couple splicing to environmental signals; this represents a new form of posttranslational regulation. Of particular interest is RadA from the archaeon Pyrococcus horikoshii, for which long-range intein-extein interactions block splicing, requiring temperature and single-stranded DNA (ssDNA) substrate to splice rapidly and accurately. Here, we report that splicing of the intein-containing RadA from another archaeon, Thermococcus sibericus, is activated by significantly lower temperatures than is P. horikoshii RadA, consistent with differences in their growth environments. Investigati

SUBMITTER: Lennon CW 

PROVIDER: S-EPMC5790916 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature