Spatial distributions of phosphorylated membrane proteins aquaporin 0 and MP20 across young and aged human lenses.

ABSTRACT: In the human ocular lens it is now realized that post-translational modifications can alter protein function and/or localization in fiber cells that no longer synthesize proteins. The specific sites of post-translational modification to the abundant ocular lens membrane proteins AQP0 and MP20 have been previously identified and their functional effects are emerging. To further understand how changes in protein function and/or localization induced by these modifications alter lens homeostasis, it is necessary to determine the spatial distributions of these modifications across the lens. In this study, a quantitative LC-MS approach was used to determine the spatial distributions of phosphorylated AQP0 and MP20 peptides from manually dissected, concentric layers of fiber cells from young and

SUBMITTER: Gutierrez DB 

PROVIDER: S-EPMC5791148 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature