Reactive astrocytes potentiate tumor aggressiveness in a murine glioma resection and recurrence model.

ABSTRACT:

Background

Surgical resection is a universal component of glioma therapy. Little is known about the postoperative microenvironment due to limited preclinical models. Thus, we sought to develop a glioma resection and recurrence model in syngeneic immune-competent mice to understand how surgical resection influences tumor biology and the local microenvironment.

Methods

We genetically engineered cells from a murine glioma mouse model to express fluorescent and bioluminescent reporters. Established allografts were resected using image-guided microsurgery. Postoperative tumor recurrence was monitored by serial imaging, and the peritumoral microenvironment was characterized by histopathology and immunohistochemistry. Coculture techniques were used to explore how astrocyte injury i

SUBMITTER: Okolie O 

PROVIDER: S-EPMC5791515 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature