Metabarcoding analysis of eukaryotic microbiota in the gut of HIV-infected patients.

ABSTRACT: Research on the relationship between changes in the gut microbiota and human disease, including AIDS, is a growing field. However, studies on the eukaryotic component of the intestinal microbiota have just begun and have not yet been conducted in HIV-infected patients. Moreover, eukaryotic community profiling is influenced by the use of different methodologies at each step of culture-independent techniques. Herein, initially, four DNA extraction protocols were compared to test the efficiency of each method in recovering eukaryotic DNA from fecal samples. Our results revealed that recovering eukaryotic components from fecal samples differs significantly among DNA extraction methods. Subsequently, the composition of the intestinal eukaryotic microbiota was evaluated in HIV-infected patients

SUBMITTER: Hamad I 

PROVIDER: S-EPMC5791994 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature