Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000_RAD and 15000_RAD.

ABSTRACT: The availability of genomic resources including linkage information for camelids has been very limited. Here, we describe the construction of a set of two radiation hybrid (RH) panels (5000_RAD and 15000_RAD) for the dromedary (Camelus dromedarius) as a permanent genetic resource for camel genome researchers worldwide. For the 5000_RAD panel, a total of 245 female camel-hamster radiation hybrid clones were collected, of which 186 were screened with 44 custom designed marker loci distributed throughout camel genome. The overall mean retention frequency (RF) of the final set of 93 hybrids was 47.7%. For the 15000_RAD panel, 238 male dromedary-hamster radiation hybrid clones were collected, of which 93 were tested using 44 PCR markers. The final set of

SUBMITTER: Perelman PL 

PROVIDER: S-EPMC5792482 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature