Blockade of ACK1/TNK2 To Squelch the Survival of Prostate Cancer Stem-like Cells.

ABSTRACT: Prostate cancer stem-like cells (PCSCs) are not only enriched in the CD44⁺PSA^-/lo subpopulation but also employ androgen-independent signaling mechanisms for survival. CD44⁺ PCSCs defy androgen deprivation, resist chemo- and radiotherapy and are highly tumorigenic. Human prostate tissue microarray (TMA) staining revealed an increased membranous staining of CD44 in the luminal compartment in higher grade G7-G9 tumors versus staining of the basal layer in benign hyperplasia. To uncover tyrosine kinase/s critical for the survival of the CD44⁺PSA^-/lo subpopulation, we performed an unbiased screen targeting 87 tyrosine kinases with gene specific siRNAs. Among a subset of tyrosine kinases crucial for PCSC survival, was a non-receptor tyrosin

SUBMITTER: Mahajan NP 

PROVIDER: S-EPMC5792546 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature