Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies.

ABSTRACT: In the past few years, many studies investigated the anaerobic digestion microbiome by means of 16S rRNA amplicon sequencing. Results obtained from these studies were compared to each other without taking into consideration the followed procedure for amplicons preparation and data analysis. This negligence was mainly due to the lack of knowledge regarding the biases influencing specific steps of the microbiome investigation process. In the present study, the main technical aspects of the 16S rRNA analysis were checked giving special attention to the approach used for high throughput sequencing. More specifically, the microbial compositions of three laboratory scale biogas reactors were analyzed before and after addition of sodium oleate by sequencing the microbiome with three different app

SUBMITTER: Campanaro S 

PROVIDER: S-EPMC5792648 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature