Transcription rate strongly affects splicing fidelity and cotranscriptionality in budding yeast.

ABSTRACT: The functional consequences of alternative splicing on altering the transcription rate have been the subject of intensive study in mammalian cells but less is known about effects of splicing on changing the transcription rate in yeast. We present several lines of evidence showing that slow RNA polymerase II elongation increases both cotranscriptional splicing and splicing efficiency and that faster elongation reduces cotranscriptional splicing and splicing efficiency in budding yeast, suggesting that splicing is more efficient when cotranscriptional. Moreover, we demonstrate that altering the RNA polymerase II elongation rate in either direction compromises splicing fidelity, and we reveal that splicing fidelity depends largely on intron length together with secondary structure and splice

SUBMITTER: Aslanzadeh V 

PROVIDER: S-EPMC5793784 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature