MinION-based long-read sequencing and assembly extends the Caenorhabditis elegans reference genome.

ABSTRACT: Advances in long-read single molecule sequencing have opened new possibilities for 'benchtop' whole-genome sequencing. The Oxford Nanopore Technologies MinION is a portable device that uses nanopore technology that can directly sequence DNA molecules. MinION single molecule long sequence reads are well suited for de novo assembly of complex genomes as they facilitate the construction of highly contiguous physical genome maps obviating the need for labor-intensive physical genome mapping. Long sequence reads can also be used to delineate complex chromosomal rearrangements, such as those that occur in tumor cells, that can confound analysis using short reads. Here, we assessed MinION long-read-derived sequences for feasibility concerning: (1) the de novo assembly of a large complex genome, a

SUBMITTER: Tyson JR 

PROVIDER: S-EPMC5793790 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature