Mapping protein interactions of sodium channel Na_V1.7 using epitope-tagged gene-targeted mice.

ABSTRACT: The voltage-gated sodium channel Na_V1.7 plays a critical role in pain pathways. We generated an epitope-tagged Na_V1.7 mouse that showed normal pain behaviours to identify channel-interacting proteins. Analysis of Na_V1.7 complexes affinity-purified under native conditions by mass spectrometry revealed 267 proteins associated with Nav1.7 in vivo The sodium channel β3 (Scn3b), rather than the β1 subunit, complexes with Nav1.7, and we demonstrate an interaction between collapsing-response mediator protein (Crmp2) and Nav1.7, through which the analgesic drug lacosamide regulates Nav1.7 current density. Novel Na_V1.7 protein interactors including membrane-trafficking protein synaptotagmin-2 (Syt2), L-type amino acid transporter 1 (Lat1) and trans

SUBMITTER: Kanellopoulos AH 

PROVIDER: S-EPMC5793798 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature