Evaluation of a microfluidic flow assay to screen for von Willebrand disease and low von Willebrand factor levels.

ABSTRACT: Essentials von Willebrand factor (VWF) function is shear stress dependent. Platelet accumulation in a microfluidic assay correlates with VWF levels. The microfluidic assay discriminates type 1 von Willebrand disease from healthy controls. The microfluidic flow assay detects responses to therapeutic intervention (DDAVP).

Summary

Background von Willebrand disease (VWD) is a mucocutaneous bleeding disorder with a reported prevalence of 1 in 10 000. von Willebrand factor (VWF) function and platelet adhesion are regulated by hemodynamic forces that are not integrated into most current clinical assays. Objective We evaluated whether a custom microfluidic flow assay (MFA) can screen for deficiencies in VWF in patients presenting with mucocutaneous bleeding. Methods Whole blood from individ

SUBMITTER: Lehmann M 

PROVIDER: S-EPMC5794217 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature