Diverse functions of homologous actin isoforms are defined by their nucleotide, rather than their amino acid sequence.

ABSTRACT: β- and γ-cytoplasmic actin are nearly indistinguishable in their amino acid sequence, but are encoded by different genes that play non-redundant biological roles. The key determinants that drive their functional distinction are unknown. Here, we tested the hypothesis that β- and γ-actin functions are defined by their nucleotide, rather than their amino acid sequence, using targeted editing of the mouse genome. Although previous studies have shown that disruption of β-actin gene critically impacts cell migration and mouse embryogenesis, we demonstrate here that generation of a mouse lacking β-actin protein by editing β-actin gene to encode γ-actin protein, and vice versa, does not affect cell migration and/or organism survival. Our data suggest that the essential in vivo function of β-actin

SUBMITTER: Vedula P 

PROVIDER: S-EPMC5794254 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature