A putative RNA binding protein from Plasmodium vivax apicoplast.

ABSTRACT: Malaria is caused by Apicomplexa protozoans from the Plasmodium genus entering the bloodstream of humans and animals through the bite of the female mosquitoes. The annotation of the Plasmodium vivax genome revealed a putative RNA binding protein (apiRBP) that was predicted to be trafficked into the apicoplast, a plastid organelle unique to Apicomplexa protozoans. Although a 3D structural model of the apiRBP corresponds to a noncanonical RNA recognition motif with an additional C-terminal α-helix (α₃), preliminary protein production trials were nevertheless unsuccessful. Theoretical solvation analysis of the apiRBP model highlighted an exposed hydrophobic region clustering α₃. Hence, we used a C-terminal GFP-fused chimera to stabilize the highly insoluble

SUBMITTER: Garcia-Maurino SM 

PROVIDER: S-EPMC5794462 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature