Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation.

ABSTRACT: Magnesium has been shown to effectively prevent vascular calcification associated with chronic kidney disease. Magnesium has been hypothesized to prevent the upregulation of osteoblastic genes that potentially drives calcification. However, extracellular effects of magnesium on hydroxyapatite formation are largely neglected. This study investigated the effects of magnesium on intracellular changes associated with transdifferentiation and extracellular crystal formation. Bovine vascular smooth muscle cells were calcified using β-glycerophosphate. Transcriptional analysis, alkaline phosphatase activity and detection of apoptosis were used to identify transdifferentiation. Using X-ray diffraction and energy dispersive spectroscopy extracellular crystal composition was investigated. Magnesium

SUBMITTER: Ter Braake AD 

PROVIDER: S-EPMC5794996 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature