Enhanced Wort Fermentation with De Novo Lager Hybrids Adapted to High-Ethanol Environments.

ABSTRACT: Interspecific hybridization is a valuable tool for developing and improving brewing yeast in a number of industry-relevant aspects. However, the genomes of newly formed hybrids can be unstable. Here, we exploited this trait by adapting four brewing yeast strains, three of which were de novo interspecific lager hybrids with different ploidy levels, to high ethanol concentrations in an attempt to generate variant strains with improved fermentation performance in high-gravity wort. Through a batch fermentation-based adaptation process and selection based on a two-step screening process, we obtained eight variant strains which we compared to the wild-type strains in 2-liter-scale wort fermentations replicating industrial conditions. The results revealed that the adapted variants outperf

SUBMITTER: Krogerus K 

PROVIDER: S-EPMC5795086 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature