Transient Expression and Purification of Horseradish Peroxidase C in Nicotiana benthamiana.

ABSTRACT: Horseradish peroxidase (HRP) is a commercially important reagent enzyme used in molecular biology and in the diagnostic product industry. It is typically purified from the roots of the horseradish (Armoracia rusticana); however, this crop is only available seasonally, yields are variable and often low, and the product is a mixture of isoenzymes. Engineering high-level expression in transiently transformed tobacco may offer a solution to these problems. In this study, a synthetic Nicotiana benthamiana codon-adapted full-length HRP isoenzyme gene as well as C-terminally truncated and both N- and C-terminally truncated versions of the HRP C gene were synthesized, and their expression in N. benthamiana was evaluated using an Agrobacterium tumefaciens-m

SUBMITTER: Huddy SM 

PROVIDER: S-EPMC5796064 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature