Peptide-Mediated Liposome Fusion: The Effect of Anchor Positioning.

ABSTRACT: A minimal model system for membrane fusion, comprising two complementary peptides dubbed "E" and "K" joined to a cholesterol anchor via a polyethyleneglycol spacer, has previously been developed in our group. This system promotes the fusion of large unilamellar vesicles and facilitates liposome-cell fusion both in vitro and in vivo. Whilst several aspects of the system have previously been investigated to provide an insight as to how fusion is facilitated, anchor positioning has not yet been considered. In this study, the effects of placing the anchor at either the N-terminus or in the center of the peptide are investigated using a combination of circular dichroism spectroscopy, dynamic light scattering, and fluorescence assays. It was discovered that anchoring the "K" peptide in the cente

SUBMITTER: Crone NSA 

PROVIDER: S-EPMC5796160 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature