Evaluation of Nanobody Conjugates and Protein Fusions as Bioanalytical Reagents.

ABSTRACT: Enzyme-linked immunosorbent assay (ELISA), flow cytometry, and Western blot are common bioanalytical techniques. Successful execution traditionally requires the use of one or more commercially available antibody-small-molecule dyes or antibody-reporter protein conjugates that recognize relatively short peptide tags (<15 amino acids). However, the size of antibodies and their molecular complexity (by virtue of post-translational disulfide formation and glycosylation) typically require either expression in mammalian cells or purification from immunized mammals. The preparation and purification of chemical dye- or reporter protein-antibody conjugates is often complicated and expensive and not commonplace in academic laboratories. In response, researchers have developed comparatively simpler p

SUBMITTER: Bruce VJ 

PROVIDER: S-EPMC5796518 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature