Comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis.

ABSTRACT: The successful completion of cytokinesis requires the coordinated activities of diverse cellular components including membranes, cytoskeletal elements and chromosomes that together form partly redundant pathways, depending on the cell type. The biochemical analysis of this process is challenging due to its dynamic and rapid nature. Here, we systematically compared monopolar and bipolar cytokinesis and demonstrated that monopolar cytokinesis is a good surrogate for cytokinesis and it is a well-suited system for global biochemical analysis in mammalian cells. Based on this, we established a phosphoproteomic signature of cytokinesis. More than 10,000 phosphorylation sites were systematically monitored; around 800 of those were up-regulated during cytokinesis. Reconstructing the kinase-substra

SUBMITTER: Karayel O 

PROVIDER: S-EPMC5797227 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature