Crawling-induced floor dust resuspension affects the microbiota of the infant breathing zone.

ABSTRACT:

Background

Floor dust is commonly used for microbial determinations in epidemiological studies to estimate early-life indoor microbial exposures. Resuspension of floor dust and its impact on infant microbial exposure is, however, little explored. The aim of our study was to investigate how floor dust resuspension induced by an infant's crawling motion and an adult walking affects infant inhalation exposure to microbes.

Results

We conducted controlled chamber experiments with a simplified mechanical crawling infant robot and an adult volunteer walking over carpeted flooring. We applied bacterial 16S rRNA gene sequencing and quantitative PCR to monitor the infant breathing zone microbial content and compared that to the adult breathing zone and the carpet dust as the source. D

SUBMITTER: Hyytiainen HK 

PROVIDER: S-EPMC5797336 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature