Clinical value of miR-198-5p in lung squamous cell carcinoma assessed using microarray and RT-qPCR.

ABSTRACT:

Background

To examine the clinical value of miR-198-5p in lung squamous cell carcinoma (LUSC).

Methods

Gene Expression Omnibus (GEO) microarray datasets were used to explore the miR-198-5p expression and its diagnostic value in LUSC. Real-time reverse transcription quantitative polymerase chain reaction was used to evaluate the expression of miR-198-5p in 23 formalin-fixed, paraffin-embedded (FFPE) LUSC tissues and corresponding non-cancerous tissues. The correlation between miR-198-5p expression and clinic pathological features was assessed. Meanwhile, putative target messenger RNAs of miR-198-5p were identified based on the analysis of differentially expressed genes in the Cancer Genome Atlas (TCGA) and 12 miRNA prediction tools. Subsequently, the putative target genes wer

SUBMITTER: Liang YY 

PROVIDER: S-EPMC5797354 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature