Methylation of the genes ROD1, NLRC5, and HKR1 is associated with aging in Hainan centenarians.

ABSTRACT:

Background

Human aging is a hot topic in biology, and it has been associated with DNA methylation changes at specific genomic sites. We aimed to study the changes of DNA methylation at a single-CpG-site resolution using peripheral blood samples from centenarians.

Methods

Using Illumina 450 K Methylation BeadChip microarray assays, we carried out a pool-based, epigenome-wide investigation of DNA methylation of blood samples from 12 centenarians and 12 healthy controls. Differentially methylated cytosine-phosphate-guanosine (CpG) sites were selected for further pyrosequencing analysis of blood samples from 30 centenarians and 30 healthy controls.

Result

We identified a total of 31 high-confidence CpG sites with differential methylation profiles between the groups: 9 (29

SUBMITTER: Zeng Q 

PROVIDER: S-EPMC5797414 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature