Cadmium nanoparticles citrullinate cytokeratins within lung epithelial cells: cadmium as a potential cause of citrullination in chronic obstructive pulmonary disease.

ABSTRACT:

Objective

The objective of the study was to determine whether the cadmium-derived materials induce intracellular protein citrullination.

Methods

Human A549 lung epithelial cells were exposed to cadmium in soluble and nanoparticulate forms represented by cadmium chloride (CdCl₂) and cadmium oxide (CdO), respectively, and their combinations with ultrafine carbon black (ufCB) produced by high temperature combustion, imitating cigarette burning. Protein citrullination in cell lysates was analyzed by Western immunoblotting and verified by immunofluorescent confocal microscopy. Target citrullinated proteins were identified by proteomic analysis.

Results

CdO, ufCB and its combination with CdCl₂ and CdO after high temperature combustion induced protein c

SUBMITTER: Hutchinson D 

PROVIDER: S-EPMC5797466 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature