16-Channel Flexible System to Measure Electrophysiological Properties of Bioengineered Hearts.

ABSTRACT: As tissue engineering continues to mature, it is necessary to develop new technologies that bring insight into current paradigms and guide improvements for future experiments. To this end, we have developed a system to characterize our bioartificial heart model and compare them to functional native structures. In the present study, the hearts of adult Sprague-Dawley were decellularized resulting in a natural three-dimensional cardiac scaffold. Neonatal rat primary cardiac cells were then cultured within a complex 3D fibrin gel, forming a 3-dimensional cardiac construct, which was sutured to the acellular scaffold and suspended in media for 24-48 h. The resulting bioartificial hearts (BAHs) were then affixed with 16 electrodes, in different configurations to evaluate not only the electrocar

SUBMITTER: Salazar BH 

PROVIDER: S-EPMC5797494 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature