Motoneuron activity is required for enhancements in functional recovery after peripheral nerve injury in exercised female mice.

ABSTRACT: Inhibitory luminopsins (iLMO2) integrate opto- and chemo-genetic approaches and allow for cell-type specific inhibition of neuronal activity. When exposed to a Renilla luciferase substrate, Coelenterazine (CTZ), iLMO2 generates bioluminescence-mediated activation of its amino-terminal halorhodopsin, resulting in neuronal inhibition. Moderate daily exercise in the form of interval treadmill-training (IT) applied following a peripheral nerve injury results in enhanced motor axon regeneration and muscle fiber reinnervation in female mice. We hypothesized that iLMO2 mediated inhibition of motoneuron activity during IT would block this enhancement. Unilateral intramuscular injections of Cre-dependent AAV2/9-EF1a-DIO-iLMO2 (∼8.5 x 10¹³ vg/ml) were made into the gastrocnemius and tibia

SUBMITTER: Jaiswal PB 

PROVIDER: S-EPMC5797526 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature