Quantification and tracking of genetically engineered dendritic cells for studying immunotherapy.

ABSTRACT:

Purpose

Genetically encoded reporters can assist in visualizing biological processes in live organisms and have been proposed for longitudinal and noninvasive tracking of therapeutic cells in deep tissue. Cells can be labeled in situ or ex vivo and followed in live subjects over time. Nevertheless, a major challenge for reporter systems is to identify the cell population that actually expresses an active reporter.

Methods

We have used a nucleoside analog, pyrrolo-2'-deoxycytidine, as an imaging probe for the putative reporter gene, Drosophila melanogaster 2'-deoxynucleoside kinase. Bioengineered cells were imaged in vivo in animal models of brain tumor and immunotherapy using chemical exchange saturation transfer MRI. The number of transduced cells was quantified by flow cyt

SUBMITTER: Bar-Shir A 

PROVIDER: S-EPMC5797672 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature