Subcellular Localization and Activity of TRPM4 in Medial Prefrontal Cortex Layer 2/3.

ABSTRACT: TRPM4 is a Ca²⁺-activated non-selective cationic channel that conducts monovalent cations. TRPM4 has been proposed to contribute to burst firing and sustained activity in several brain regions, however, the cellular and subcellular pattern of TRPM4 expression in medial prefrontal cortex (mPFC) during postnatal development has not been elucidated. Here, we use multiplex immunofluorescence labeling of brain sections to characterize the postnatal developmental expression of TRPM4 in the mouse mPFC. We also performed electrophysiological recordings to correlate the expression of TRPM4 immunoreactivity with the presence of TRPM4-like currents. We found that TRPM4 is expressed from the first postnatal day, with expression increasing up to postnatal day 35. Additionally, in perforated

SUBMITTER: Riquelme D 

PROVIDER: S-EPMC5797675 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature