In vivo evidence for possible up-regulating roles of lysophosphatidic acid around fertilization in rats.

ABSTRACT: Lysophosphatidic acid (LPA) produced by autotaxin (ATX) is recognized as a multi-functional mediator in mammalian reproduction. This study focused on possible effect(s) of LPA on ovulated cumulus-oocyte complexes (COCs) around fertilization in rats in vivo. Immunohistochemistry revealed the cell-type-dependent localization of candidates of synthetic enzymes, ATX and two phospholipases A₂ isofroms, and LPA receptors LPA_1-4 in ovulated COCs and in oviductal epithelium. The eggs ovulated with a form of COCs became denuded of cumulus cells and underwent fragmentation in the absence of fertilization. In vivo experiments of local administration in non-copulated rats demonstrated that eggs denudation was increased by LPA and decreased by anti-ATX antibody and that fragmentat

SUBMITTER: Takeda T 

PROVIDER: S-EPMC5797857 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature