MicroRNA Profiling of Transgenic Mice with Myocardial Overexpression of Nucleolin.

ABSTRACT:

Background

Nucleolin (NCL) is the most abundant RNA-binding protein in the cell nucleolus and plays an important role in chromatin stability, ribosome assembly, ribosomal RNA maturation, ribosomal DNA transcription, nucleocytoplasmic transport, and regulation of RNA stability and translation efficiency. In addition to its anti-apoptotic properties, the underlying mechanisms associated with NCL-related roles in different cellular processes remain unclear. In this study, the effect of NCL on microRNA (miRNA) expression was evaluated by generating transgenic mice with myocardial overexpression of NCL and by analyzing microarrays of mature and precursor miRNAs from mice.

Methods

Using microinjection of alpha-MyHc clone 26-NCL plasmids, we generated transgenic mice with myocardia

SUBMITTER: Lyu QL 

PROVIDER: S-EPMC5798056 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature